GENETIC TEACHER

SSCP Marker consider co-dominant inheritance marker with multi-allelic nature and doesn't require prior sequence information and provide genetic background variation for functional analysis and genetic map studies, SSCP Marker consider sensitive marker with low frequent polymorphism and require intensive labor with time consuming, SSCP Marker use in molecular diagnosis studies and mutation detection and gene typing and genetic maps (Watch Related Video in #geneticteacher) #geneticteacher

Single Strand Conformation Polymorphism or SSCP Marker is an electrophoretic PCR marker study difference of single strand DNA mobility through non-denture polyacrylamide gel to detect point mutations and other small scale changes based on primary nucleotide sequence, SSCP Marker initiate by DNA extraction for PCR amplification using specific combination of forward and reverse primers and denature double strand PCR product to single strand using heat or chemicals and run non-denature polyacrylamide gel electrophoresis to define mutations according to fragment size and sequence information (Watch Related Video in #geneticteacher) #geneticteacher

Single Strand Conformation Polymorphism or SSCP Marker is an electrophoretic PCR marker study difference of single strand DNA mobility through non-denture polyacrylamide gel to detect point mutations and other small scale changes based on primary nucleotide sequence, SSCP Marker initiate by DNA extraction for PCR amplification using specific combination of forward and reverse primers and denature double strand PCR product to single strand using heat or chemicals and run non-denature polyacrylamide gel electrophoresis to define mutations according to fragment size and sequence information, SSCP Marker consider co-dominant inheritance marker with multi-allelic nature and doesn't require prior sequence information and provide genetic background variation for functional analysis and genetic map studies, SSCP Marker consider sensitive marker with low frequent polymorphism and require intensive labor with time consuming, SSCP Marker use in molecular diagnosis studies and mutation detecti...

STS Marker consider co-dominant inheritance marker and stable and reproducible and informative marker and act as universal genetic marker for physical maps in different Labs to construct physical map, STS Marker consider high sensitive marker and require time consuming with high technical skills, STS Marker use in population studies and genetic physical maps and genetic linkage maps and genome mapping (Watch Related Video in #geneticteacher) #geneticteacher

Sequence-tagged Site or STS Marker is short DNA sequence (200 to 500) bp in genome detect by PCR to define single sequence tagged site that flanking by specific PCR primer, STS Marker initiate by DNA extraction for PCR amplification using specific forward and reverse primer that bind to ends of tagged site and demonstrate specificity of PCR reaction by gel electrophoresis to construct physical map (Watch Related Video in #geneticteacher) #geneticteacher

Sequence-tagged Site or STS Marker is short DNA sequence (200 to 500) bp in genome detect by PCR to define single sequence tagged site that flanking by specific PCR primer, STS Marker initiate by DNA extraction for PCR amplification using specific forward and reverse primer that bind to ends of tagged site and demonstrate specificity of PCR reaction by gel electrophoresis to construct physical map, STS Marker consider co-dominant inheritance marker and stable and reproducible and informative marker and act as universal genetic marker for physical maps in different Labs to construct physical map, STS Marker consider high sensitive marker and require time consuming with high technical skills, STS Marker use in population studies and genetic physical maps and genetic linkage maps and genome mapping (Watch Related Video in #geneticteacher) #geneticteacher

SSLP Marker consider co-dominant inheritance marker with multi-allelic nature and classify as a stable and reproducible and informative marker, SSLP Marker costly if interested primers not available and have scoring errors if alternation in primer annealing site found, SSLP Marker use in genetic diversity and DNA fingerprinting and Marker Assisted Selection and genetic linkage map and cloning studies (Watch Related Video in #geneticteacher) #geneticteacher

SSLP Marker initiate by DNA extraction for PCR amplification using combination of forward and reverse primers based on sequence of flanking regions and separate amplified PCR product by gel electrophoresis to score present (1) and absent (0) bands (Watch Related Video in #geneticteacher) #geneticteacher

Simple Sequence Length Polymorphism or SSLP Marker is a microsatellite PCR marker similar to SSR marker array repeat unique sequence of genomic DNA to display length of allelic variation that contains different numbers of unique repeat unit, SSLP Marker mostly occur in non-coding regions and control gene expression of coding regions without coding any protein in form of (Di-, Tri-, Tetra- and Penta-nucleotide repeats) (Watch Related Video in #geneticteacher) #geneticteacher

Simple Sequence Length Polymorphism or SSLP Marker is a microsatellite PCR marker similar to SSR marker array repeat unique sequence of genomic DNA to display length of allelic variation that contains different numbers of unique repeat unit, SSLP Marker mostly occur in non-coding regions and control gene expression of coding regions without coding any protein in form of (Di-, Tri-, Tetra- and Penta-nucleotide repeats), SSLP Marker initiate by DNA extraction for PCR amplification using combination of forward and reverse primers based on sequence of flanking regions and separate amplified PCR product by gel electrophoresis to score present (1) and absent (0) bands, SSLP Marker consider co-dominant inheritance marker with multi-allelic nature and classify as a stable and reproducible and informative marker, SSLP Marker costly if interested primers not available and have scoring errors if alternation in primer annealing site found, SSLP Marker use in genetic diversity and DNA fingerprint...

DArT Marker consider Bi-allelic nature marker without require prior sequence information in identifying numerous of polymorphic markers in parallel and suitable with Mendelian inheritance, DArT Marker is a dominant inheritance marker with sensitivity to methylation and require extensive investment and skills in labs, DArT Marker use in genetic diversity and population structure and association genetics and genetic linkage maps and marker assisted selections studies (Watch Related Video in #geneticteacher) #geneticteacher

DArT Marker initiate by reduce DNA complexity for genome representation using combination of restriction enzymes and ligate restriction fragments to adaptor and PCR amplification by primers with complementary sequences to adapter and Clone representative fragments into vector in suitable host and select bacterial colonies based on antibiotic resistance in X-gal medium and amplify inserts clones by using vector specific primers and purify and array amplified product on microarray system and use DArT software and fluorescent probes to detect polymorphisms, The observed polymorphism result from single nucleotide substitution within restriction sites or Indel in insertion sites or differences in methylation status (Watch Related Video in #geneticteacher) #geneticteacher

Diversity Arrays Technology (DArT Marker) is highly variable genome wide binary marker based on restriction site polymorphism for DNA hybridization on microarray system to discover polymorphic fragments, Diversity Arrays Technology Sequencing (DArT-Seq) use next generation sequencing to test unknown sequence samples to detect allelic variations for genotyping and other genetic analysis (Watch Related Video in #geneticteacher) #geneticteacher

Diversity Arrays Technology (DArT Marker) is highly variable genome wide binary marker based on restriction site polymorphism for DNA hybridization on microarray system to discover polymorphic fragments, Diversity Arrays Technology Sequencing (DArT-Seq) use next generation sequencing to test unknown sequence samples to detect allelic variations for genotyping and other genetic analysis, DArT Marker initiate by reduce DNA complexity for genome representation using combination of restriction enzymes and ligate restriction fragments to adaptor and PCR amplification by primers with complementary sequences to adapter and Clone representative fragments into vector in suitable host and select bacterial colonies based on antibiotic resistance in X-gal medium and amplify inserts clones by using vector specific primers and purify and array amplified product on microarray system and use DArT software and fluorescent probes to detect polymorphisms, The observed polymorphism result from single nu...

In comparison between SNP Marker and InDel Marker the SN Marker change single nucleotide of DNA sequence while InDel Marker insert or delete one or more nucleotides between same or closely related species (Watch Related Video in #geneticteacher) #geneticteacher

InDel Marker consider co-dominant inheritance marker with bi-allelic nature across whole genome and high accuracy and good stability in kinship studies, IndDl Marker use high resolution (4-6)% agarose gel to distinguish small amplified DNA fragments, InDel Marker use in genetic diversity and germplasm management and genetic structure of large population and kinship analysis and fine gene mapping (Watch Related Video in #geneticteacher) #geneticteacher

Insertion-Deletion Marker or InDel Marker is a DNA marker screen functional genes related to important traits between same or closely related species by screening errors in sequence replication or screening insertion of transposable elements or screening un-equal crossing over, InDel Marker initiate by DNA extraction for PCR amplification based on specific forward and reverse primers and separate amplified PCR product by agarose gel electrophoresis to score present (1) or absent (0) specific bands (Watch Related Video in #geneticteacher) #geneticteacher

Insertion-Deletion Marker or InDel Marker is a DNA marker screen functional genes related to important traits between same or closely related species by screening errors in sequence replication or screening insertion of transposable elements or screening un-equal crossing over, InDel Marker initiate by DNA extraction for PCR amplification based on specific forward and reverse primers and separate amplified PCR product by agarose gel electrophoresis to score present (1) or absent (0) specific bands, InDel Marker consider co-dominant inheritance marker with bi-allelic nature across whole genome and high accuracy and good stability in kinship studies, IndDl Marker use high resolution (4-6)% agarose gel to distinguish small amplified DNA fragments, InDel Marker use in genetic diversity and germplasm management and genetic structure of large population and kinship analysis and fine gene mapping, Finally In comparison between SNP Marker and InDel Marker the SN Marker change single nucleotid...

TRAP marker consider co-dominant inheritance marker with multi-allelic nature and classify as stable and reproducible and informative marker by identifying location of genes that control interested traits, TRAP marker require prior sequence information (EST sequence) and require combination of fixed and arbitrary primers to enhance detection efficiency, TRAP marker use in genetic diversity and population structure and genetic linkage maps and gene tagging for interesting traits and cloning studies (Watch Related Video in #geneticteacher) #geneticteacher

Target region amplification polymorphism TRAP marker is an efficient PCR marker improved from SRAP marker use available information of DNA sequence to detect target genes by using fixed and arbitrary primers, TRAP marker initiate by DNA extraction for PCR amplification using fixed and arbitrary primers and separate PCR product by gel electrophoresis to score present (1) or absent (0) Bands by using known sequence of candidate genes (EST sequence) as fixed primer with arbitrary primer which rich with adenine and thymine or cytosine and guanine to amplify target genes (Watch Related Video in #geneticteacher) #geneticteacher

Target region amplification polymorphism TRAP marker is an efficient PCR marker improved from SRAP marker use available information of DNA sequence to detect target genes by using fixed and arbitrary primers, TRAP marker initiate by DNA extraction for PCR amplification using fixed and arbitrary primers and separate PCR product by gel electrophoresis to score present (1) or absent (0) Bands by using known sequence of candidate genes (EST sequence) as fixed primer with arbitrary primer which rich with adenine and thymine or cytosine and guanine to amplify target genes, TRAP marker consider co-dominant inheritance marker with multi-allelic nature and classify as stable and reproducible and informative marker by identifying location of genes that control interested traits, TRAP marker require prior sequence information (EST sequence) and require combination of fixed and arbitrary primers to enhance detection efficiency, TRAP marker use in genetic diversity and population structure and gen...

SRAP marker consider co-dominant inheritance marker with multi-allelic nature and classify as stable and reproducible and informative marker, SRAP marker require prior sequence information of samples and require set of primer combinations to enhance detection efficiency, SRAP marker use in genetic diversity and population structure and genetic linkage maps and gene tagging and cloning studies (Watch Related Video in #geneticteacher) #geneticteacher

SRAP marker initiate by DNA extraction for PCR amplification using forward and reverse primers and separate PCR product by agarose gel electrophoresis to score present (1) and absent (0) bands, SRAP Forward primers consist of unspecific filler sequence and guanine and cytosine sequence to target guanine and cytosine rich regions as exons and selective nucleotide, SRAP Reverse primers consist of unspecific filler sequence and adenine and thymine sequence to target adenine and thymine rich regions as promoter and introns and selective nucleotide (Watch Related Video in #geneticteacher) #geneticteacher

Sequence related amplified polymorphism or SRAP marker amplify open reading frames (ORFs) by target promoter and exons and introns regions in whole genome to link actual genes, SRAP marker initiate by DNA extraction for PCR amplification using forward and reverse primers and separate PCR product by agarose gel electrophoresis to score present (1) and absent (0) bands (Watch Related Video in #geneticteacher) #geneticteacher

Sequence related amplified polymorphism or SRAP marker amplify open reading frames (ORFs) by target promoter and exons and introns regions in whole genome to link actual genes, SRAP marker initiate by DNA extraction for PCR amplification using forward and reverse primers and separate PCR product by agarose gel electrophoresis to score present (1) and absent (0) bands, SRAP Forward primers consist of unspecific filler sequence and guanine and cytosine sequence to target guanine and cytosine rich regions as exons and selective nucleotide, SRAP Reverse primers consist of unspecific filler sequence and adenine and thymine sequence to target adenine and thymine rich regions as promoter and introns and selective nucleotide, SRAP marker consider co-dominant inheritance marker with multi-allelic nature and classify as stable and reproducible and informative marker, SRAP marker require prior sequence information of samples and require set of primer combinations to enhance detection efficiency,...