Principle of CAPS and dCAPS Markers

DArT Marker initiate by reduce DNA complexity for genome representation using combination of restriction enzymes and ligate restriction fragments to adaptor and PCR amplification by primers with complementary sequences to adapter and Clone representative fragments into vector in suitable host and select bacterial colonies based on antibiotic resistance in X-gal medium and amplify inserts clones by using vector specific primers and purify and array amplified product on microarray system and use DArT software and fluorescent probes to detect polymorphisms, The observed polymorphism result from single nucleotide substitution within restriction sites or Indel in insertion sites or differences in methylation status (Watch Related Video in #geneticteacher) #geneticteacher