Principle of CAPS and dCAPS Markers

Sequence related amplified polymorphism or SRAP marker amplify open reading frames (ORFs) by target promoter and exons and introns regions in whole genome to link actual genes, SRAP marker initiate by DNA extraction for PCR amplification using forward and reverse primers and separate PCR product by agarose gel electrophoresis to score present (1) and absent (0) bands, SRAP Forward primers consist of unspecific filler sequence and guanine and cytosine sequence to target guanine and cytosine rich regions as exons and selective nucleotide, SRAP Reverse primers consist of unspecific filler sequence and adenine and thymine sequence to target adenine and thymine rich regions as promoter and introns and selective nucleotide, SRAP marker consider co-dominant inheritance marker with multi-allelic nature and classify as stable and reproducible and informative marker, SRAP marker require prior sequence information of samples and require set of primer combinations to enhance detection efficiency, SRAP marker use in genetic diversity and population structure and genetic linkage maps and gene tagging and cloning studies (Watch Related Video in #geneticteacher) #geneticteacher