Principle of CAPS and dCAPS Markers

Diversity Arrays Technology (DArT Marker) is highly variable genome wide binary marker based on restriction site polymorphism for DNA hybridization on microarray system to discover polymorphic fragments, Diversity Arrays Technology Sequencing (DArT-Seq) use next generation sequencing to test unknown sequence samples to detect allelic variations for genotyping and other genetic analysis, DArT Marker initiate by reduce DNA complexity for genome representation using combination of restriction enzymes and ligate restriction fragments to adaptor and PCR amplification by primers with complementary sequences to adapter and Clone representative fragments into vector in suitable host and select bacterial colonies based on antibiotic resistance in X-gal medium and amplify inserts clones by using vector specific primers and purify and array amplified product on microarray system and use DArT software and fluorescent probes to detect polymorphisms, The observed polymorphism result from single nucleotide substitution within restriction sites or Indel in insertion sites or differences in methylation status, DArT Marker consider Bi-allelic nature marker without require prior sequence information in identifying numerous of polymorphic markers in parallel and suitable with Mendelian inheritance, DArT Marker is a dominant inheritance marker with sensitivity to methylation and require extensive investment and skills in labs, DArT Marker use in genetic diversity and population structure and association genetics and genetic linkage maps and marker assisted selections studies (Watch Related Video in #geneticteacher) #geneticteacher