Principle of CAPS and dCAPS Markers

Insertion-Deletion Marker or InDel Marker is a DNA marker screen functional genes related to important traits between same or closely related species by screening errors in sequence replication or screening insertion of transposable elements or screening un-equal crossing over, InDel Marker initiate by DNA extraction for PCR amplification based on specific forward and reverse primers and separate amplified PCR product by agarose gel electrophoresis to score present (1) or absent (0) specific bands, InDel Marker consider co-dominant inheritance marker with bi-allelic nature across whole genome and high accuracy and good stability in kinship studies, IndDl Marker use high resolution (4-6)% agarose gel to distinguish small amplified DNA fragments, InDel Marker use in genetic diversity and germplasm management and genetic structure of large population and kinship analysis and fine gene mapping, Finally In comparison between SNP Marker and InDel Marker the SN Marker change single nucleotide of DNA sequence while InDel Marker insert or delete one or more nucleotides between same or closely related species (Watch Related Video in #geneticteacher) #geneticteacher