Principle of CAPS and dCAPS Markers

Sequence-tagged Site or STS Marker is short DNA sequence (200 to 500) bp in genome detect by PCR to define single sequence tagged site that flanking by specific PCR primer, STS Marker initiate by DNA extraction for PCR amplification using specific forward and reverse primer that bind to ends of tagged site and demonstrate specificity of PCR reaction by gel electrophoresis to construct physical map (Watch Related Video in #geneticteacher) #geneticteacher