Principle of CAPS and dCAPS Markers

Target region amplification polymorphism TRAP marker is an efficient PCR marker improved from SRAP marker use available information of DNA sequence to detect target genes by using fixed and arbitrary primers, TRAP marker initiate by DNA extraction for PCR amplification using fixed and arbitrary primers and separate PCR product by gel electrophoresis to score present (1) or absent (0) Bands by using known sequence of candidate genes (EST sequence) as fixed primer with arbitrary primer which rich with adenine and thymine or cytosine and guanine to amplify target genes (Watch Related Video in #geneticteacher) #geneticteacher