Principle of CAPS and dCAPS Markers

Target region amplification polymorphism TRAP marker is an efficient PCR marker improved from SRAP marker use available information of DNA sequence to detect target genes by using fixed and arbitrary primers, TRAP marker initiate by DNA extraction for PCR amplification using fixed and arbitrary primers and separate PCR product by gel electrophoresis to score present (1) or absent (0) Bands by using known sequence of candidate genes (EST sequence) as fixed primer with arbitrary primer which rich with adenine and thymine or cytosine and guanine to amplify target genes, TRAP marker consider co-dominant inheritance marker with multi-allelic nature and classify as stable and reproducible and informative marker by identifying location of genes that control interested traits, TRAP marker require prior sequence information (EST sequence) and require combination of fixed and arbitrary primers to enhance detection efficiency, TRAP marker use in genetic diversity and population structure and genetic linkage maps and gene tagging for interesting traits and cloning studies (Watch Related Video in #geneticteacher) #geneticteacher