Principle of CAPS and dCAPS Markers

Single Strand Conformation Polymorphism or SSCP Marker is an electrophoretic PCR marker study difference of single strand DNA mobility through non-denture polyacrylamide gel to detect point mutations and other small scale changes based on primary nucleotide sequence, SSCP Marker initiate by DNA extraction for PCR amplification using specific combination of forward and reverse primers and denature double strand PCR product to single strand using heat or chemicals and run non-denature polyacrylamide gel electrophoresis to define mutations according to fragment size and sequence information, SSCP Marker consider co-dominant inheritance marker with multi-allelic nature and doesn't require prior sequence information and provide genetic background variation for functional analysis and genetic map studies, SSCP Marker consider sensitive marker with low frequent polymorphism and require intensive labor with time consuming, SSCP Marker use in molecular diagnosis studies and mutation detection and gene typing and genetic maps (Watch Related Video in #geneticteacher) #geneticteacher