Principle of CAPS and dCAPS Markers

Simple Sequence Length Polymorphism or SSLP Marker is a microsatellite PCR marker similar to SSR marker array repeat unique sequence of genomic DNA to display length of allelic variation that contains different numbers of unique repeat unit, SSLP Marker mostly occur in non-coding regions and control gene expression of coding regions without coding any protein in form of (Di-, Tri-, Tetra- and Penta-nucleotide repeats), SSLP Marker initiate by DNA extraction for PCR amplification using combination of forward and reverse primers based on sequence of flanking regions and separate amplified PCR product by gel electrophoresis to score present (1) and absent (0) bands, SSLP Marker consider co-dominant inheritance marker with multi-allelic nature and classify as a stable and reproducible and informative marker, SSLP Marker costly if interested primers not available and have scoring errors if alternation in primer annealing site found, SSLP Marker use in genetic diversity and DNA fingerprinting and Marker Assisted Selection and genetic linkage map and cloning studies (Watch Related Video in #geneticteacher) #geneticteacher