Principle of CAPS and dCAPS Markers

SRAP marker initiate by DNA extraction for PCR amplification using forward and reverse primers and separate PCR product by agarose gel electrophoresis to score present (1) and absent (0) bands, SRAP Forward primers consist of unspecific filler sequence and guanine and cytosine sequence to target guanine and cytosine rich regions as exons and selective nucleotide, SRAP Reverse primers consist of unspecific filler sequence and adenine and thymine sequence to target adenine and thymine rich regions as promoter and introns and selective nucleotide (Watch Related Video in #geneticteacher) #geneticteacher