GENETIC TEACHER

SAGE Technology provide quantitative and comprehensive gene expression profile in a given cell sample and identify novel transcripts without prior sequence information, SAGE Technology consider labor and time consuming technology without identify 5’ ends of mRNA which lead to sequence errors, SAGE Technology use in Biology and Medicine and Pharmacology studies for global gene expression analysis and discover novel transcripts (Watch Related Video in #geneticteacher) #geneticteacher

SAGE Technology provide quantitative and comprehensive gene expression profile in a given cell sample and identify novel transcripts without prior sequence information, SAGE Technology consider labor and time consuming technology without identify 5’ ends of mRNA which lead to sequence errors, SAGE Technology use in Biology and Medicine and Pharmacology studies for global gene expression analysis and discover novel transcripts (Watch Related Video in #geneticteacher) #geneticteacher

SAGE Technology initiate by isolate mRNA and add biotin label thiamin primer to synthesize cDNA and bind product to streptavidin coat beads and cleave with anchor enzyme and discard loos fragments and divide product into two pools and add linker sequences for ligation and leave product with tag enzyme to combine pools and ligate and amplify ditags and cleave with anchor enzyme to ligate ditags for sequencing and recording tags and frequencies, SAGE Technology require sequencing techniques and computer software's and bioinformatics knowledge (Watch Related Video in #geneticteacher) #geneticteacher

Serial Analysis of Gene Expression or SAGE Technology is a transcriptom technique produce snapshot of mRNA of particular sample in form of short sequence tags (10-14) bp that correspond to fragments of those transcripts by linkage tags serially into long cDNA for efficient cloning and sequencing to quantify global gene expression in given cell (Watch Related Video in #geneticteacher) #geneticteacher

Serial Analysis of Gene Expression or SAGE Technology is a transcriptom technique produce snapshot of mRNA of particular sample in form of short sequence tags (10-14) bp that correspond to fragments of those transcripts by linkage tags serially into long cDNA for efficient cloning and sequencing to quantify global gene expression in given cell, SAGE Technology initiate by isolate mRNA and add biotin label thiamin primer to synthesize cDNA and bind product to streptavidin coat beads and cleave with anchor enzyme and discard loos fragments and divide product into two pools and add linker sequences for ligation and leave product with tag enzyme to combine pools and ligate and amplify ditags and cleave with anchor enzyme to ligate ditags for sequencing and recording tags and frequencies, SAGE Technology require sequencing techniques and computer software's and bioinformatics knowledge, SAGE Technology provide quantitative and comprehensive gene expression profile in a given cell sample...

EST Marker consider co-dominant inheritance marker and fast and efficient method of gene expression profile and provide sequence information for microarray construction because it have 100% coding sequence, EST Marker consider labor and time consuming method and don't yield full sequence of mRNA and contain sequence errors and relative frequent chimeric sequence, EST Marker use in gene expression and gene discovery and gene prediction and coding sequence maps studies (Watch Related Video in #geneticteacher) #geneticteacher

Expressed Sequence Tags or EST Marker is a short DNA sequence (200 – 500) bp generate by single pass sequencing of cDNA clones to identify gene expression profile in cell at particular time, EST Marker initiate by RNA extraction from particular tissue and treat extracted RNA with reverse transcriptase to generate cDNA and clone cDNA in suitable host (E.coli) to construct cDNA library and randomly select clones for PCR and sequencing and examine finding EST with database to find matching sequence (Watch Related Video in #geneticteacher) #geneticteacher

Expressed Sequence Tags or EST Marker is a short DNA sequence (200 – 500) bp generate by single pass sequencing of cDNA clones to identify gene expression profile in cell at particular time, EST Marker initiate by RNA extraction from particular tissue and treat extracted RNA with reverse transcriptase to generate cDNA and clone cDNA in suitable host (E.coli) to construct cDNA library and randomly select clones for PCR and sequencing and examine finding EST with database to find matching sequence, EST Marker consider co-dominant inheritance marker and fast and efficient method of gene expression profile and provide sequence information for microarray construction because it have 100% coding sequence, EST Marker consider labor and time consuming method and don't yield full sequence of mRNA and contain sequence errors and relative frequent chimeric sequence, EST Marker use in gene expression and gene discovery and gene prediction and coding sequence maps studies (Watch Related Video i...

SCoT Marker consider multi-locus and efficient and informative marker because of polymorphism directly related to gene function based on transcribed regions of genome without require prior sequence information, SCoT Marker consider dominant marker and it's primer length and annealing temperature aren't sole factors for reproducibility, SCoT Marker use for mapping functional genes and gene tagging and genetic diversity and DNA fingerprinting and kinship relationship studies (Watch Related Video in #geneticteacher) #geneticteacher

Start Codon Target Polymorphism or SCoT Marker is a simple gene target marker based on short conserved region surround translation start codon (ATG) to track inheritance of functional genes, SCoT Marker initiate by DNA extraction for PCR amplification using long single universal primer (18-mer) to flank (ATG) region on two strand of DNA and separate amplified PCR product by agarose gel electrophoresis to score present (1) or absent (0) bands (Watch Related Video in #geneticteacher) #geneticteacher

Start Codon Target Polymorphism or SCoT Marker is a simple gene target marker based on short conserved region surround translation start codon (ATG) to track inheritance of functional genes, SCoT Marker initiate by DNA extraction for PCR amplification using long single universal primer (18-mer) to flank (ATG) region on two strand of DNA and separate amplified PCR product by agarose gel electrophoresis to score present (1) or absent (0) bands, SCoT Marker consider multi-locus and efficient and informative marker because of polymorphism directly related to gene function based on transcribed regions of genome without require prior sequence information, SCoT Marker consider dominant marker and it's primer length and annealing temperature aren't sole factors for reproducibility, SCoT Marker use for mapping functional genes and gene tagging and genetic diversity and DNA fingerprinting and kinship relationship studies (Watch Related Video in #geneticteacher) #geneticteacher

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SCAR Marker consider co-dominant inheritance marker with locus specific nature and stable and reproducible and informative marker, SCAR Marker require skills and efforts and expense in designing specific primer for each locus and exhibit dominance when primer partially overlap site of sequence variation, SCAR Marker use for locus specificity and gene tagging and DNA fingerprinting and genetic diversity and marker assisted selection (Watch Related Video in #geneticteacher) #geneticteacher

Direct SCAR Marker initiate by DNA extraction for PCR amplification of RAPDs assay and separate amplified PCR product by gel electrophoresis and cut and purify interested fragment from agarose gel for sequencing and compare sequence using BLAST tool in NCBI website to synthesize specific SCAR primers that flank regions of original RAPDs primers and re-amplify template with SCAR primers to Identify specific Bands, Indirect SCAR Marker initiate by DNA extraction for PCR amplification of RAPDs assay and separate amplified PCR product by gel electrophoresis and cut and purify interested fragment from agarose gel and clone purified interested fragment in suitable vector and transform vector to high component cells of E-coli bacteria to isolate plasmid DNA from selected transformed colonies and digest plasmid DNA by restriction enzymes to check the same size fragment that corresponding to RAPDs assay fragment for sequencing to synthesize specific SCAR primers that flank regions of original R...

Indirect SCAR Marker initiate by DNA extraction for PCR amplification of RAPDs assay and separate amplified PCR product by gel electrophoresis and cut and purify interested fragment from agarose gel and clone purified interested fragment in suitable vector and transform vector to high component cells of E-coli bacteria to isolate plasmid DNA from selected transformed colonies and digest plasmid DNA by restriction enzymes to check the same size fragment that corresponding to RAPDs assay fragment for sequencing to synthesize specific SCAR primers that flank regions of original RAPDs primers and re-amplify template with SCAR primers to Identify specific Bands (Watch Related Video in #geneticteacher) #geneticteacher

Direct SCAR Marker initiate by DNA extraction for PCR amplification of RAPDs assay and separate amplified PCR product by gel electrophoresis and cut and purify interested fragment from agarose gel for sequencing and compare sequence using BLAST tool in NCBI website to synthesize specific SCAR primers that flank regions of original RAPDs primers and re-amplify template with SCAR primers to Identify specific Bands (Watch Related Video in #geneticteacher) #geneticteacher

Sequence Characterized Amplified Region or SCAR Marker or Locus Specific Marker is a PCR-agarose gel electrophoresis based on sequence specific polymorphic RAPDs fragments use cloning and sequencing techniques to synthesize specific primers (16-24) bp in length to identify specific locus that linked to interested traits (Watch Related Video in #geneticteacher) #geneticteacher

Sequence Characterized Amplified Region or SCAR Marker or Locus Specific Marker is a PCR-agarose gel electrophoresis based on sequence specific polymorphic RAPDs fragments use cloning and sequencing techniques to synthesize specific primers (16-24) bp in length to identify specific locus that linked to interested traits, Direct SCAR Marker initiate by DNA extraction for PCR amplification of RAPDs assay and separate amplified PCR product by gel electrophoresis and cut and purify interested fragment from agarose gel for sequencing and compare sequence using BLAST tool in NCBI website to synthesize specific SCAR primers that flank regions of original RAPDs primers and re-amplify template with SCAR primers to Identify specific Bands, Indirect SCAR Marker initiate by DNA extraction for PCR amplification of RAPDs assay and separate amplified PCR product by gel electrophoresis and cut and purify interested fragment from agarose gel and clone purified interested fragment in suitable vector and...

derived Cleaved Amplified Polymorphic Sequence or d-CAPS Marker is a modification of CAPS Marker based on SNP marker use mismatch primer with one or two base change to perform PCR and restriction enzyme digestion and separate digested PCR fragments by gel electrophoresis to score present (1) or absent (0) bands (Watch Related Video in #geneticteacher) #geneticteacher

CAPS Marker consider Co-dominant inheritance marker with locus specific nature and classify as stable and reproducible and informative marker, CAPS Marker have a limited sized of amplified fragments which difficult in scoring multi-gene families and require sequence data for primer synthesis for each locus, CAPS Marker use in genetic diversity and population structure and marker assisted selection and genome mapping and gene tagging (Watch Related Video in #geneticteacher) #geneticteacher

Cleaved Amplified Polymorphic Sequence or CAPS Marker is a PCR-RFLP marker generate polymorphisms at a particular locus according to restriction sites or restriction fragment length caused by SNP or InDel Markers, CAPS Marker initiate by DNA extraction for PCR amplification of target Loci by using specific primers and digest PCR product with appropriate restriction enzymes and separate digested PCR product by gel electrophoresis to score present (1) and absent (0) specific bands (Watch Related Video in #geneticteacher) #geneticteacher

Cleaved Amplified Polymorphic Sequence or CAPS Marker is a PCR-RFLP marker generate polymorphisms at a particular locus according to restriction sites or restriction fragment length caused by SNP or InDel Markers, CAPS Marker initiate by DNA extraction for PCR amplification of target Loci by using specific primers and digest PCR product with appropriate restriction enzymes and separate digested PCR product by gel electrophoresis to score present (1) and absent (0) specific bands, CAPS Marker consider Co-dominant inheritance marker with locus specific nature and classify as stable and reproducible and informative marker, CAPS Marker have a limited sized of amplified fragments which difficult in scoring multi-gene families and require sequence data for primer synthesis for each locus, CAPS Marker use in genetic diversity and population structure and marker assisted selection and genome mapping and gene tagging, derived Cleaved Amplified Polymorphic Sequence or d-CAPS Marker is a modifica...