Principle of CAPS and dCAPS Markers

Start Codon Target Polymorphism or SCoT Marker is a simple gene target marker based on short conserved region surround translation start codon (ATG) to track inheritance of functional genes, SCoT Marker initiate by DNA extraction for PCR amplification using long single universal primer (18-mer) to flank (ATG) region on two strand of DNA and separate amplified PCR product by agarose gel electrophoresis to score present (1) or absent (0) bands, SCoT Marker consider multi-locus and efficient and informative marker because of polymorphism directly related to gene function based on transcribed regions of genome without require prior sequence information, SCoT Marker consider dominant marker and it's primer length and annealing temperature aren't sole factors for reproducibility, SCoT Marker use for mapping functional genes and gene tagging and genetic diversity and DNA fingerprinting and kinship relationship studies (Watch Related Video in #geneticteacher) #geneticteacher