Principle of CAPS and dCAPS Markers

Expressed Sequence Tags or EST Marker is a short DNA sequence (200 – 500) bp generate by single pass sequencing of cDNA clones to identify gene expression profile in cell at particular time, EST Marker initiate by RNA extraction from particular tissue and treat extracted RNA with reverse transcriptase to generate cDNA and clone cDNA in suitable host (E.coli) to construct cDNA library and randomly select clones for PCR and sequencing and examine finding EST with database to find matching sequence, EST Marker consider co-dominant inheritance marker and fast and efficient method of gene expression profile and provide sequence information for microarray construction because it have 100% coding sequence, EST Marker consider labor and time consuming method and don't yield full sequence of mRNA and contain sequence errors and relative frequent chimeric sequence, EST Marker use in gene expression and gene discovery and gene prediction and coding sequence maps studies (Watch Related Video in #geneticteacher) #geneticteacher