Principle of CAPS and dCAPS Markers

Serial Analysis of Gene Expression or SAGE Technology is a transcriptom technique produce snapshot of mRNA of particular sample in form of short sequence tags (10-14) bp that correspond to fragments of those transcripts by linkage tags serially into long cDNA for efficient cloning and sequencing to quantify global gene expression in given cell, SAGE Technology initiate by isolate mRNA and add biotin label thiamin primer to synthesize cDNA and bind product to streptavidin coat beads and cleave with anchor enzyme and discard loos fragments and divide product into two pools and add linker sequences for ligation and leave product with tag enzyme to combine pools and ligate and amplify ditags and cleave with anchor enzyme to ligate ditags for sequencing and recording tags and frequencies, SAGE Technology require sequencing techniques and computer software's and bioinformatics knowledge, SAGE Technology provide quantitative and comprehensive gene expression profile in a given cell sample and identify novel transcripts without prior sequence information, SAGE Technology consider labor and time consuming technology without identify 5’ ends of mRNA which lead to sequence errors, SAGE Technology use in Biology and Medicine and Pharmacology studies for global gene expression analysis and discover novel transcripts, SAGE Technology have many modification which are (Micro-SAGE + Small Amplified RNA-SAGE + Mini-SAGE + Long-SAGE + Robust-Long SAGE + Super-SAGE) (Watch Related Video in #geneticteacher) #geneticteacher