Principle of CAPS and dCAPS Markers

SAGE Technology initiate by isolate mRNA and add biotin label thiamin primer to synthesize cDNA and bind product to streptavidin coat beads and cleave with anchor enzyme and discard loos fragments and divide product into two pools and add linker sequences for ligation and leave product with tag enzyme to combine pools and ligate and amplify ditags and cleave with anchor enzyme to ligate ditags for sequencing and recording tags and frequencies, SAGE Technology require sequencing techniques and computer software's and bioinformatics knowledge (Watch Related Video in #geneticteacher) #geneticteacher