Principle of CAPS and dCAPS Markers

Direct SCAR Marker initiate by DNA extraction for PCR amplification of RAPDs assay and separate amplified PCR product by gel electrophoresis and cut and purify interested fragment from agarose gel for sequencing and compare sequence using BLAST tool in NCBI website to synthesize specific SCAR primers that flank regions of original RAPDs primers and re-amplify template with SCAR primers to Identify specific Bands, Indirect SCAR Marker initiate by DNA extraction for PCR amplification of RAPDs assay and separate amplified PCR product by gel electrophoresis and cut and purify interested fragment from agarose gel and clone purified interested fragment in suitable vector and transform vector to high component cells of E-coli bacteria to isolate plasmid DNA from selected transformed colonies and digest plasmid DNA by restriction enzymes to check the same size fragment that corresponding to RAPDs assay fragment for sequencing to synthesize specific SCAR primers that flank regions of original RAPDs primers and re-amplify template with SCAR primers to Identify specific Bands (Watch Related Video in #geneticteacher) #geneticteacher