Principle of CAPS and dCAPS Markers

Cleaved Amplified Polymorphic Sequence or CAPS Marker is a PCR-RFLP marker generate polymorphisms at a particular locus according to restriction sites or restriction fragment length caused by SNP or InDel Markers, CAPS Marker initiate by DNA extraction for PCR amplification of target Loci by using specific primers and digest PCR product with appropriate restriction enzymes and separate digested PCR product by gel electrophoresis to score present (1) and absent (0) specific bands (Watch Related Video in #geneticteacher) #geneticteacher