Principle of CAPS and dCAPS Markers

derived Cleaved Amplified Polymorphic Sequence or d-CAPS Marker is a modification of CAPS Marker based on SNP marker use mismatch primer with one or two base change to perform PCR and restriction enzyme digestion and separate digested PCR fragments by gel electrophoresis to score present (1) or absent (0) bands (Watch Related Video in #geneticteacher) #geneticteacher