Principle of CAPS and dCAPS Markers

Cleaved Amplified Polymorphic Sequence or CAPS Marker is a PCR-RFLP marker generate polymorphisms at a particular locus according to restriction sites or restriction fragment length caused by SNP or InDel Markers, CAPS Marker initiate by DNA extraction for PCR amplification of target Loci by using specific primers and digest PCR product with appropriate restriction enzymes and separate digested PCR product by gel electrophoresis to score present (1) and absent (0) specific bands, CAPS Marker consider Co-dominant inheritance marker with locus specific nature and classify as stable and reproducible and informative marker, CAPS Marker have a limited sized of amplified fragments which difficult in scoring multi-gene families and require sequence data for primer synthesis for each locus, CAPS Marker use in genetic diversity and population structure and marker assisted selection and genome mapping and gene tagging, derived Cleaved Amplified Polymorphic Sequence or d-CAPS Marker is a modification of CAPS Marker based on SNP marker use mismatch primer with one or two base change to perform PCR and restriction enzyme digestion and separate digested PCR fragments by gel electrophoresis to score present (1) or absent (0) bands (Watch Related Video in #geneticteacher) #geneticteacher