Principle of CAPS and dCAPS Markers

Start Codon Target Polymorphism or SCoT Marker is a simple gene target marker based on short conserved region surround translation start codon (ATG) to track inheritance of functional genes, SCoT Marker initiate by DNA extraction for PCR amplification using long single universal primer (18-mer) to flank (ATG) region on two strand of DNA and separate amplified PCR product by agarose gel electrophoresis to score present (1) or absent (0) bands (Watch Related Video in #geneticteacher) #geneticteacher