GENETIC TEACHER

Significant of Whole Genome Sequencing and Target or Gene Sequencing

Steps of DNA Sequencing

Types of DNA Sequencing

Types and Steps of DNA Sequencing

Principle of DNA Sequencing

Principle and Steps of DNA Sequencing

Principle and Types of DNA Sequencing

Principle and Types and Steps of DNA Sequencing

DNA Sequencing

First Generation Sequencing regarding Maxam and Gilbert and Sanger sequencing methods, Second and Third Generations Sequencing regarding next generation platforms ( Illumina and PacBio and Nanopore and others such as Mini Seq and Next Seq 500 and Hi Seq 2500 and Hi Seq 3000 and Hi Seq 4000, etc.), Gaps refer to un-sequenced regions of DNA, Contigs refer to continues sequence of assembled DNA, Coverage refer to number of times that sequencer cover DNA sequence (Watch Related Video in #geneticteacher) #geneticteacher

DNA Sequencing study genes structure and functions without require prior information and identify responsible genes for interested traits and detect mutations, DNA Sequencing consider labor and costly and time consuming method and require skills in computer algorithms, DNA Sequencing use in genetic diversity and DNA fingerprinting and gene manipulation and editing and metagenomic and evolutionary relationship studies (Watch Related Video in #geneticteacher) #geneticteacher

DNA Sequencing initiate by DNA extraction and DNA fragmentation into smaller pieces by restriction enzymes and adaptor ligation to each ends of DNA fragments for library preparation and library enrichment by PCR to generate quantities of DNA fragments with adaptors for sequencing by using any of sequence platforms and align sequences to reference genomes (Watch Related Video in #geneticteacher) #geneticteacher

Whole Genome Sequencing is type of DNA sequencing determine complete DNA sequences in genome at single time (Chromosomes + Mitochondria + Chloroplast in plants) for comprehensive analysis of entire genome and study variants in coding regions, Target Sequencing or Gene Sequencing is type of DNA sequencing determine sequence of particular genes from whole genome sequences using specific probes to capture interested genes in process of hybridization for sequencing to study genetic analysis in details, Shotgun Sequencing is type of DNA sequencing determine sequence of large piece of DNA, Clone by Clone Sequencing is type of DNA sequencing determine accurate sequence of various chromosomes at single time using vectors to generate multiple copies of each DNA fragments, Pyrosequencing is type of DNA Sequencing depending upon release of PPi and ATP (Watch Related Video in #geneticteacher) #geneticteacher

DNA Sequencing Determine precise order of nucleotides along genome by denature DNA for fragmentation and library preparation for sequencing to study genes structure and functions and genetic variation, Whole Genome Sequencing is type of DNA sequencing determine complete DNA sequences in genome at single time (Chromosomes + Mitochondria + Chloroplast in plants) for comprehensive analysis of entire genome and study variants in coding regions, Target Sequencing or Gene Sequencing is type of DNA sequencing determine sequence of particular genes from whole genome sequences using specific probes to capture interested genes in process of hybridization for sequencing to study genetic analysis in details, Shotgun Sequencing is type of DNA sequencing determine sequence of large piece of DNA, Clone by Clone Sequencing is type of DNA sequencing determine accurate sequence of various chromosomes at single time using vectors to generate multiple copies of each DNA fragments, Pyrosequencing is type o...

Significant of Single Cell RNA Sequencing

Significant of mRNA and Target RNA and Small RNA Sequencing

Significant of Direct and Indirect and Total RNA Sequencing

Advantages and Disadvantages and Applications of RNA Sequencing

Steps of RNA Sequencing

Types of RNA Sequencing

Types and Steps of RNA Sequencing

Principle and Systems of RNA Sequencing

Principle and Steps of RNA Sequencing

Principle and Types of RNA Sequencing

Principle and Types and Steps of RNA Sequencing

RNA Sequencing or Whole Transcriptome Shotgun Sequencing

Systems of RNA sequencing (I-sequence 100 system and next sequence 1000 and next sequence 2000 and nova sequence 6000 system and nova sequence X series) (Watch Related Video in #geneticteacher) #geneticteacher

RNA Sequencing detect differences in gene expression levels without prior knowledge of genome sequence to detect novel transcripts and detect allele specific expression and detect alternative gene splicing transcripts and detect post transcriptional modifications and detect gene fusions and isoforms and other structural variants, RNA Sequencing consider labor and costly and time consuming method because analyzed RNA sequencing data require significant computational resources and experts, RNA Sequencing use in mutation studies and gene expression studies and disease diagnosis studies and biomarker studies and epigenetic studies and functional genomic studies and Pharmacogenomics studies (Watch Related Video in #geneticteacher) #geneticteacher

RNA Sequencing initiate by RNA extraction and RNA selection to select target RNA and synthesize cDNA from isolated RNA and prepare cDNA library to generate millions reads of each sample in process of fragmentation to optimize sequencing of target cDNA and adaptor ligation to bind transcripts as priming site for sequencing and index and amplification by add specific sequence or barcode to transcripts during PCR amplification of cDNA to increase concentration of developed cDNA library for sequencing by next generation sequencing and analyze obtain data by bioinformatics tools by comparing obtain sequence with reference sequence (Watch Related Video in #geneticteacher) #geneticteacher

Whole transcription RNA sequencing or Total RNA sequencing sequence all types of RNAs to target both coding and non-coding transcripts to provide all information about gene expression, mRNA sequencing sequence Poly-A library (directly or in-directly) to identify expressed coding genes, Target RNA sequencing sequence specific transcript to study particular genes or pathways, Small RNA sequencing sequence microRNA and short interfering RNA and Piwi interacting RNA to study gene regulation, Single cell RNA sequencing sequence RNA from individual cells to characterize cellular heterogeneity within complex cell and identify unknown and rare cell types and explore changes in gene expression among cell type and trace trajectory of distinct cell lineage (Watch Related Video in #geneticteacher) #geneticteacher

Direct RNA sequencing perform by sequence isolated RNA directly without converting to cDNA while in-direct RNA sequencing or complementary RNA sequencing perform by sequence cDNA after converting from isolated RNA (Watch Related Video in #geneticteacher) #geneticteacher

RNA sequencing or Whole Transcriptome Shotgun Sequencing is next generation sequencing method provide snapshot of coding and non-coding transcripts to understand connection between genome and its gene expression and identify novel features of gene fusions and isoforms, Direct RNA sequencing perform by sequence isolated RNA directly without converting to cDNA while in-direct RNA sequencing or complementary RNA sequencing perform by sequence cDNA after converting from isolated RNA, Whole transcription RNA sequencing or Total RNA sequencing sequence all types of RNAs to target both coding and non-coding transcripts to provide all information about gene expression, mRNA sequencing sequence Poly-A library (directly or in-directly) to identify expressed coding genes, Target RNA sequencing sequence specific transcript to study particular genes or pathways, Small RNA sequencing sequence microRNA and short interfering RNA and Piwi interacting RNA to study gene regulation, Single cell RNA sequen...

Advantages and Disadvantages and Applications of Microarrays Technology

Microscopic Slide Spots Colors of DNA Microarrays Technology

Steps of DNA Microarrays Technology

Principle and Types and Requirements of DNA Microarrays Technology

Principle and Types and Requirements and Steps of DNA Microarrays Technology

Principle and Types of Microarrays Technology

Microarrays Technology

Microarrays Technology provide data of thousands of genes expression and identify new genes and their function and expression levels under different conditions, Microarrays Technology consider labor and costly and time consuming method because it need technical skills for huge amount of data, Microarrays Technology use in genome wide analysis of gene expression and disease diagnosis and drug discovery and development and biomarker discovery and toxicological studies (Watch Related Video in #geneticteacher) #geneticteacher

Example of DNA microarrays between normal and cancer cells (Green Spot mean gene expressed in normal cells only) / (Red Spot mean gene expressed in cancer cells only) (Yellow Spot mean gene expressed in both normal and cancer cells) / (Black Spot mean gene not expressed in any cells) (Watch Related Video in #geneticteacher) #geneticteacher

DNA Microarrays Technology initiate by isolate mRNA from test sample and make cDNA to label with red fluorescent by CY5 dye and isolate mRNA from control sample and make cDNA to label with green fluorescent by CY3 dye and mix cDNA together to hybridize cDNA with array that corresponding gene sequence and wash un-hybridized cDNA to remove unbound sequence to easy read array with laser by scanner for data analysis, Example of DNA microarrays between normal and cancer cells (Green Spot mean gene expressed in normal cells only) / (Red Spot mean gene expressed in cancer cells only) (Yellow Spot mean gene expressed in both normal and cancer cells) / (Black Spot mean gene not expressed in any cells) (Watch Related Video in #geneticteacher) #geneticteacher

DNA Microarrays Technology array thousands of genes by microscopic slide spots and each spot contain known DNA sequence as probe that complementary to specific genes to determine (ON/OFF) genes, DNA Microarrays Technology have four types (cDNA Microarrays + Oligo DNA Microarrays + BAC Microarrays + SNP Microarrays), DNA Microarrays Technology require (DNA chip + Target Sample + Fluorescent dyes + Probes + Scanner), DNA Microarrays Technology initiate by isolate mRNA from test sample and make cDNA to label with red fluorescent by CY5 dye and isolate mRNA from control sample and make cDNA to label with green fluorescent by CY3 dye and mix cDNA together to hybridize cDNA with array that corresponding gene sequence and wash un-hybridized cDNA to remove unbound sequence to easy read array with laser by scanner for data analysis, Example of DNA microarrays between normal and cancer cells (Green Spot mean gene expressed in normal cells only) / (Red Spot mean gene expressed in cancer cel...

DNA Microarrays detect genetic variation based on genomic sequence, RNA Microarrays detect gene expression based on known sequences, Protein Microarrays detect protein interaction based on protein sequence, Chromosomal Microarrays detect imbalance of DNA copy number (Watch Related Video in #geneticteacher) #geneticteacher