Principle of CAPS and dCAPS Markers

RNA Sequencing initiate by RNA extraction and RNA selection to select target RNA and synthesize cDNA from isolated RNA and prepare cDNA library to generate millions reads of each sample in process of fragmentation to optimize sequencing of target cDNA and adaptor ligation to bind transcripts as priming site for sequencing and index and amplification by add specific sequence or barcode to transcripts during PCR amplification of cDNA to increase concentration of developed cDNA library for sequencing by next generation sequencing and analyze obtain data by bioinformatics tools by comparing obtain sequence with reference sequence (Watch Related Video in #geneticteacher) #geneticteacher