First Generation Sequencing regarding Maxam and Gilbert and Sanger sequencing methods, Second and Third Generations Sequencing regarding next generation platforms ( Illumina and PacBio and Nanopore and others such as Mini Seq and Next Seq 500 and Hi Seq 2500 and Hi Seq 3000 and Hi Seq 4000, etc.), Gaps refer to un-sequenced regions of DNA, Contigs refer to continues sequence of assembled DNA, Coverage refer to number of times that sequencer cover DNA sequence (Watch Related Video in #geneticteacher)
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