RNA Sequencing detect differences in gene expression levels without prior knowledge of genome sequence to detect novel transcripts and detect allele specific expression and detect alternative gene splicing transcripts and detect post transcriptional modifications and detect gene fusions and isoforms and other structural variants, RNA Sequencing consider labor and costly and time consuming method because analyzed RNA sequencing data require significant computational resources and experts, RNA Sequencing use in mutation studies and gene expression studies and disease diagnosis studies and biomarker studies and epigenetic studies and functional genomic studies and Pharmacogenomics studies (Watch Related Video in #geneticteacher) #geneticteacher

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