Principle of CAPS and dCAPS Markers

DNA Sequencing Determine precise order of nucleotides along genome by denature DNA for fragmentation and library preparation for sequencing to study genes structure and functions and genetic variation, Whole Genome Sequencing is type of DNA sequencing determine complete DNA sequences in genome at single time (Chromosomes + Mitochondria + Chloroplast in plants) for comprehensive analysis of entire genome and study variants in coding regions, Target Sequencing or Gene Sequencing is type of DNA sequencing determine sequence of particular genes from whole genome sequences using specific probes to capture interested genes in process of hybridization for sequencing to study genetic analysis in details, Shotgun Sequencing is type of DNA sequencing determine sequence of large piece of DNA, Clone by Clone Sequencing is type of DNA sequencing determine accurate sequence of various chromosomes at single time using vectors to generate multiple copies of each DNA fragments, Pyrosequencing is type of DNA Sequencing depending upon release of PPi and ATP, DNA Sequencing initiate by DNA extraction and DNA fragmentation into smaller pieces by restriction enzymes and adaptor ligation to each ends of DNA fragments for library preparation and library enrichment by PCR to generate quantities of DNA fragments with adaptors for sequencing by using any of sequence platforms and align sequences to reference genomes, DNA Sequencing study genes structure and functions without require prior information and identify responsible genes for interested traits and detect mutations, DNA Sequencing consider labor and costly and time consuming method and require skills in computer algorithms, DNA Sequencing use in genetic diversity and DNA fingerprinting and gene manipulation and editing and metagenomic and evolutionary relationship studies, First Generation Sequencing regarding Maxam and Gilbert and Sanger sequencing methods, Second and Third Generations Sequencing regarding next generation platforms ( Illumina and PacBio and Nanopore and others such as Mini Seq and Next Seq 500 and Hi Seq 2500 and Hi Seq 3000 and Hi Seq 4000, etc.), Gaps refer to un-sequenced regions of DNA, Contigs refer to continues sequence of assembled DNA, Coverage refer to number of times that sequencer cover DNA sequence (Watch Related Video in #geneticteacher) #geneticteacher