Principle of CAPS and dCAPS Markers

Cell Spreader Tool is a microbiology tool distribute and spread liquid bacterial cells evenly over agar surface plate without gouging or cutting medium to achieve uniform colony growth of bacterial cells which makes it easier to quantify bacteria in solution, Reusable Cell Spreader Tool is a glass or metal stainless steel tool sterilize at high temperature for next use, Disposable Plastic Spreader Tool use directly at once without sterilization and discard into biohazard container to avoid cross-contamination and all of these types have various shapes which are (L-shape / T-shape / Triangular-shape), The work steps of Cell Spreader Tool initiate by make series dilution of target bacterial samples and place agar plate on clean and stable surface and pipette out small amount of diluted bacterial cell suspension onto center of agar surface plate and dip blade of reusable spreader into alcohol and flame blade of reusable spreader over Bunsen burner and after that allow reusable spreader to cool before contact agar and gently start spread (Swirly) in circular pattern across center of agar surface plate to distribute cells evenly without scratching medium and rotate plate slightly during spreading to cover entire surface more evenly to ensure uniform distribution and once liquid fully absorbed (Stop Spreading) and close lid promptly to avoid contamination, Cell Spreader Tool consider Fast and Simple and Easy Bacterial Culture Method by facilitating efficient of bacterial cell distribution and preparing and maintaining stock bacterial culture and isolating bacterial single colonies for colony counting and testing antibiotic susceptibility, Cell Spreader Tool doesn’t support sufficient growth of microaerophiles and anaerobes., and require liquid or easily soluble samples which often need serial dilution and require sterilization after each plate to avoid cross-contamination and chance of gouging media during spreading, Cell Spreader Tool use in microbiology studies and cell culture studies and molecular cloning studies and pharmaceutical studies and clinical studies (Watch Related Video in #geneticteacher) #geneticteacher