Principle of CAPS and dCAPS Markers

Inoculation Loops or Smear Loops or Inoculation Wand or Micro-streaker are microbiology tool consist of slender handle and small circular loop at tip to isolate and inoculate bacterial colonies and streak bacteria in plates and smear bacteria on slide, Inoculation Needles designed to pierce into solid or semi-solid media to deliver bacteria accurately into interior medium for stab inoculations and motility test and anaerobic culture and harpooning under low magnification microscope, Reusable Metal Loops and Needles sterilize in flaming Bunsen burner until becomes red hot while Disposable Plastic Loops and Needles are sterile single use without need flame and discard directly after use to reduce risk contamination, Inoculation work with Loops and Needles initiate by disinfect around base of Bunsen burner to eliminate any bacteria and hold loop or needle by using thumb and first two fingers of hand and sterilize loop or needle before and after inoculation until become red hot by using blue flam of Bunsen burner and cool loop and needle for few seconds before selecting bacterial sample, During work with Inoculation Loops and Needles must sterilize reusable loops and needles before and after use and allow sufficient cooling before touch microbial culture and dispose used plastic loops and needles in biohazard containers to prevent cross contamination and regularly inspect loops and needles for any damage, Inoculation Loops and Needles transfer microbial cultures aseptically between media and streak agar plates for colonies isolation and inoculate broth cultures for microbial growth studies and perform antibiotic sensitivity and purity tests and subculture microbial strains for routine analysis, Reusable Loops and Needles are costlier and Disposable plastic Loops and Needles are quite expensive and there are high potential error and contamination during work with multiple samples, Inoculation Loops and Needles use in microbiology studies and pathogenic detection studies and molecular biology studies and cell biology studies and Immunology studies (Watch Related Video in #geneticteacher) #geneticteacher