Principle of CAPS and dCAPS Markers

Next Generations Sequencing Method or High Throughout Sequencing Method is modern sequencing platforms sequence millions of nucleic acid fragments in parallel by break target nucleic acid into smaller pieces and attach adaptor to each fragment for sequencing, Next Generations Sequencing Method use in whole genome sequencing to sequence entire genome for complete DNA sequences and whole exome sequencing to sequence all exons in genome and target sequencing to sequence specific genes and RNA sequencing or transcriptome sequencing to sequence all RNA for gene expression profile and single cell sequencing to sequence single cell to understand biological processes and epigenetic sequencing to examine modifications that affect gene activity without altering DNA sequencing e.g. DNA methylation and proteomic sequencing to enable precise protein quantification and highly multiplex sequencing to process multiple samples in single run and chip sequencing to investigate protein DNA interaction, Next Generations Sequencing Method initiate by DNA or RNA extraction and library preparation in process of fragmentation by fragment DNA or RNA into shorter sequences (100-300) bp and ligate adaptors in both ends of fragments in process of indexing or barcoding and cluster generation by binding fragments to flow cell and undergo bridge amplification to form clusters for sequencing by using massively parallel sequencing platforms and data analysis by align output sequence to reference sequence, Next Generations Sequencing Method consider fast and scalable and accurate and effective sequencing method for large scale projects without require prior knowledge of genome to study genetic variations and transcriptional variations and genetic relationship, Next Generations Sequencing Method consider labor and costly method because it require computer capacity and experts in data analysis and statistics, Next Generations Sequencing Method use in large scale genomic studies and biological and medical studies and agriculture and environmental studies and pharmacogenomics studies and forensic studies (Watch Related Video in #geneticteacher) #geneticteacher