Principle of CAPS and dCAPS Markers

Manual Sanger Sequencing Method initiate by DNA extraction and denature DNA to obtain single DNA fragments and divide DNA sample into four tubes with primer and dNTP, and Taq DNA polymerase and one ddNTPs in each tubes to terminate DNA synthesis at specific base to create set of DNA fragments with different length after that separate amplified fragments in parallel lanes (A / G / C / T) in denature polyacrylamide gel electrophoresis and autoradiography gel in X-ray film or UV light to visualize length of amplified fragments and read bands from bottom to top according to direction of DNA synthesis (5’→3’) to compare sequence with reference sequence (Watch Related Video in #geneticteacher) #geneticteacher