Principle of CAPS and dCAPS Markers

Sanger Sequencing Method or Chain Termination Sequencing Method is first generation sequencing method use Dideoxy nucleotides (ddNTPs) that lack (3-OH) hydroxyl group and incorporate with growing DNA strand during PCR reaction as DNA chain terminators to generate fragments with ends ddATP / ddGTP / ddTTP / ddCTP, dNTPs contains (3-OH) hydroxyl group while ddNTPs contains hydrogen instead of (3-OH) hydroxyl group (Watch Related Video in #geneticteacher) #geneticteacher