Principle of CAPS and dCAPS Markers

Automatic Sanger Sequencing Method fluorescently label primer at 5’ end to amplify specific DNA regions and label each of ddNTPs with fluorescent dyes and run reaction in single lane in denature polyacrylamide gel electrophoresis or Capillary gel to visualize nucleotides peaks that reflect sequence of chain termination to compare output sequence with reference sequence (Watch Related Video in #geneticteacher) #geneticteacher