Principle of CAPS and dCAPS Markers

Microsatellite markers repeat (1-7) bp with allele size (100-400) bp such as STRs, SSRs and ISSRs, Steps of fluorescent microsatellite markers initiate by PCR amplification using fluorescently labeled primer and mix product with size marker and separate product by capillary electrophoresis to detect larger and smaller allelic fragments and detect heterozygous and homozygous alleles as well, Microsatellite markers have four types: (Perfect microsatellite markers + Imperfect microsatellite markers + Compound microsatellite markers + Complex microsatellite markers), Mutation rate of microsatellite markers range between (0.001 - 0.000001) per locus per generation (Watch Related Video in #geneticteacher) #geneticteacher