Principle of CAPS and dCAPS Markers

Microsatellite markers repeat (1-7) bp with allele size (100-400) bp such as STRs, SSRs and ISSRs, Steps of fluorescent microsatellite markers initiate by PCR amplification using fluorescently labeled primer and mix product with size marker and separate product by capillary electrophoresis to detect larger and smaller allelic fragments and detect heterozygous and homozygous alleles as well, Microsatellite markers have four types: (Perfect microsatellite markers + Imperfect microsatellite markers + Compound microsatellite markers + Complex microsatellite markers), Mutation rate of microsatellite markers range between (0.001 - 0.000001) per locus per generation, Microsatellite markers depend upon length differences while SNP markers depend upon one specific different, Mini-satellite markers repeat (15-35) bp with allele size (400-1000) bp such as VNTRs, Micro and Mini-satellite markers depend upon southern blot technique by hybridization with probe to detect target sequence and PCR with pair primers to flank variable sequence, Micro and Mini-satellite markers consider co-dominant nature markers with highly polymorphic loci with less susceptible to DNA degradation to study closely related species, Micro and Mini-satellite markers use in population studies to find differences in alleles frequencies to trace specific alleles through pedigree to identify specific genes in chromosome for forensic studies (Watch Related Video in #geneticteacher) #geneticteacher