Principle of CAPS and dCAPS Markers

Inter-Simple Sequence Repeat (ISSR marker or Multi-Loci Method) is a microsatellite DNA marker use repeated sequence primers (16-25)bp length in PCR to generate (10-60) fragments as multi-loci markers between SSR sequence, There are two types of ISSR primers: (5’ anchored primer= Consist of repeated motifs with one or many non-motifs at 5’) and (3’ anchored primer= Consist of repeated motifs with one or many several non-motifs at 3’) (Watch Related Video in #geneticteacher) #geneticteacher