Principle of CAPS and dCAPS Markers

ISSR marker initiate by DNA extraction for PCR amplification using single primer of repeated DNA motifs (2-4)bp as anchors into non--repeated adjacent regions (16-18)bp to anneal to SSR sequence resulting amplification of ISSR regions that detect by agarose gel electrophoresis to score fragments as present (1) or absent (0) of particular site (Watch Related Video in #geneticteacher) #geneticteacher