Principle of CAPS and dCAPS Markers

Inter-Simple Sequence Repeat (ISSR marker or Multi-Loci Method) is a microsatellite DNA marker use repeated sequence primers (16-25)bp length in PCR to generate (10-60) fragments as multi-loci markers between SSR sequence, There are two types of ISSR primers: (5’ anchored primer= Consist of repeated motifs with one or many non-motifs at 5’) and (3’ anchored primer= Consist of repeated motifs with one or many several non-motifs at 3’), ISSR marker initiate by DNA extraction for PCR amplification using single primer of repeated DNA motifs (2-4)bp as anchors into non--repeated adjacent regions (16-18)bp to anneal to SSR sequence resulting amplification of ISSR regions that detect by agarose gel electrophoresis to score fragments as present (1) or absent (0) of particular site, ISSR marker consider highly polymorphic marker or multiple polymorphic loci without require prior knowledge of template DNA sequence to study variation with unique regions of genome at several loci for closely related species, ISSR marker classify as dominant inheritance nature marker because some primers generate poor reproducible bands with repeatability issues, ISSR marker use in genetic diversity and evolutionary biology and genetic maps and gene tagging and cloning studies (Watch Related Video in #geneticteacher) #geneticteacher