Principle of CAPS and dCAPS Markers

DNA footprinting is an in vitro DNA molecular genetic analysis study protein DNA interaction within and outside cell to determine which DNA sequence binding proteins, Steps of DNA footprinting start by amplify region of interested DNA molecule by conventional PCR and label amplified DNA with radioactivity or fluorescence label probe and apply incubation with protein and immunoprecipitation After that apply DNA modification with DNA modifiers or cleavage agents such as (DNase-1 or Hydroxyl radicals) then run polyacrylamide gel electrophoresis with uniform ladder for control sample to allow prediction of exact location of binding site so footprinting is a gap pattern on polyacrylamide gel which mean protein bound DNA and protect DNA from cleavage agent, DNA footprinting consider a powerful enough for differentiation many fragments because it can exactly locate binding site of particular ligand in comparative cheap, DNA footprinting consider less accurate and non-reproducible and hazardous by using label probes it also consider conventional and time consuming method as well, DNA footprinting used to study specific sequence of DNA-Protein interaction to study transcriptional regulation and gene expression by identifying promoter., enhancer and silence sequence of a genes (Watch Related Video in #geneticteacher) #geneticteacher