Functions of Taq DNA Polymerase in PCR Reaction

DNA fingerprinting or DNA Typing or DNA Profiling or DNA Testing Determine variation among individuals at DNA level on principle of polymorphism of DNA sequences to compare samples (Match or mismatch) such as paternity test and criminal scene, Steps of DNA fingerprinting initiate by collect samples for DNA extraction to proceed polymerase chain reaction and gel electrophoresis for bands visualization to compare matching bands, DNA fingerprinting techniques: (RFLP markers use to analyze variation and location of restriction sites) + ( AFLP markers use to analyze specific fragments of genomic DNA) + (STR markers use to analyze short repeat sequence 4 to 6 base pairs) + (VNTR markers use to analyze longer repeat sequence 10 to 100 base pairs) + (SNP markers use to examine single nucleotide variation at specific positions in genome in form of allele specific oligonucleotides) + (Mitochondrial DNA use to track maternal lineages) + (Y-chromosome use to track paternal lineages) + (Whole Genome Sequencing use to generate highly comprehensive informative genetic profile), In example of paternity test child band match father band and mismatch mother band, Child inheritance from father and mother: If father heterozygous at locus A and homozygous and locus B and mother heterozygous at each locus A and B hence child combine one allele from each parent, Example of criminal scene: if DNA profile of crime scene match suspect strong evidence that suspect was present at crime scene while if DNA profile of crime scene doesn't match suspect may be eliminate from enquiry, DNA fingerprinting use in paternity test and criminal investigations and disease identification and anthropology studies and agriculture and livestock breeding and quality control in biotechnology (Watch Related Video in #geneticteacher) #geneticteacher