Functions of Taq DNA Polymerase in PCR Reaction

DNA digest with two restriction enzymes which cut un-evenly overhang sticky ends such as., Mse-1 (Cut Frequently) and ECOR-1 (Cut Rarely), Component of restriction digestion genomic DNA in microliter: Genomic DNA and any kind of arbitrary primer (OPA) and Mse-1 and BSA (Come with Mse-1) and ECOR-1 and deionized water then vortex samples and incubate at 37 degree Celsius for 3 hours with agitate every hour after that inactivate enzymes at 70 degree Celsius for 15 minutes Next use adaptors of known sequence as priming sites to ligate sticky ends of digested DNA fragments (Watch Related Video in #geneticteacher) #geneticteacher