Functions of Taq DNA Polymerase in PCR Reaction

Component of pre-selective PCR amplification: DNA template from restriction/ligation and ECOR-1 adaptor with Adenosine nucleotide (As primer) and Mse-1 adaptor with Cytosine nucleotide (As primer) and dNTPs and 10X PCR buffer and MgCl2 and Taq DNA polymerase and deionized water, Reaction of Pre-selective PCR amplification (26 Cycles) and after finish add 100 microliter of sterile water to PCR product, After that undergo selective PCR amplification by adding two nucleotide of Adenosine and one nucleotide of Cytosine to primer at 3 ends to again select subset fragments (Be Attention) More selective nucleotide detect less polymorphism so must only add three nucleotide (Watch Related Video in #geneticteacher) #geneticteacher