Functions of Taq DNA Polymerase in PCR Reaction

Component of ECOR-1 adaptor and Mse-1 adaptor and PCR profile for each mix reagent: One selective PCR consist of adaptor sequences of each restriction enzymes and one arbitrary nucleotide (OPA) to amplify only subset of digested DNA fragments and after finish store tubes at minus 20 degree Celsius after that perform pre-selective PCR amplification to amplify specific subsets of digested DNA fragments using combination of selective primers at 3 ends which are Adenosine nucleotide to ECOR-1 adaptor and Cytosine nucleotide to Mse-1 adaptor that complementary to adaptors and restriction sites to select only subset fragments (Watch Related Video in #geneticteacher) #geneticteacher