Functions of Taq DNA Polymerase in PCR Reaction

Random Amplified Polymorphic DNA (RAPD marker) Amplify genomic DNA randomly between two identical short sequence with single short oligonucleotide primer (10 base pair) based on PCR amplification by using low annealing temperature (35 to 45 degree Celsius) to increase sequence amplification without exactly complementary to primer sequence therefore changes in DNA sequence at each locus in RAPD marker assess by presence (1) or absence (0) bands on gel Hence RAPD polymorphism occur due to base substitution at primer binding sites or indels in regions between binding sites and the mismatch between primer and DNA template results decrease amount of PCR product (Watch Related Video in #geneticteacher) #geneticteacher