Functions of Taq DNA Polymerase in PCR Reaction

Restriction Fragment Length Polymorphism or RFLP marker require pure and high molecular weight of DNA to digest DNA with restriction enzymes and detect digested DNA by southern blot technique and detect interested gene by PCR, By making DNA extraction and digest DNA with restriction enzymes or restriction endonucleases then make gel electrophoresis of DNA fragments after that transfer DNA fragments using southern blotting then hybridizing DNA with label probe and make autoradiography, RFLP marker is co-dominant inheritance because it estimate Heterozygosity and multi loci specific marker because it define two or more alleles and it isn't require prior sequence information, On the other hand RFLP marker require large amount of DNA and present low level of polymorphism in some species and present few loci per assay with time consuming and cost, RFLP marker use in genetic diversity and genetic relationship and genetic drift and selection and whole genomic mapping and gene tagging, DNA digest with restriction enzymes in different length due to deletion of DNA between restriction sites or Insertion of DNA between restriction sites or point mutations that results gain or loss of restriction sites (Watch Related Video in #geneticteacher) #geneticteacher