Ligation Mix Adaptors of AFLP Marker: ECOR-1 adaptor and Mse-1 adaptor and T4 DNA ligase 10X buffer and T4 DNA ligase units and deionized water add 10 microliters of ligation mix to 50 microliter digested DNA after that vortex samples and incubate at room temperature for 3 hours with agitate every hour (Watch Related Video in #geneticteacher) #geneticteacher

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