Principle of CAPS and dCAPS Markers

(PCR-RAPD Marker) Initial denaturation start at 94 degree Celsius for 10 minutes and denaturation temperature should be at 94 degree Celsius for 1 minute and annealing temperature should be at 37 to 45 degree Celsius for1 minute and extension temperature should be at 72 degree Celsius for 1 minute and final extension should be at 72 degree Celsius for 10 minutes and cooling temperature should be at 4 degree Celsius forever and Polymorphic band must be clone and make sequence to design new primers to amplify specifically (Watch Related Video in #geneticteacher) #geneticteacher