Functions of Taq DNA Polymerase in PCR Reaction

PCR contents have main four components (Deionized water + PCR Mix + 10 Pico mole forward primer and 10 Pico mole reverse primer + DNA template) and after complete PCR reaction DNA samples load in wells of agarose gel from cathode to anode to show different sizes of samples, Agarose preparation (Add 1% of agarose powder + Melt agarose in 0.5 X TBE buffer + After cooling to 50% add 1 microliter ethidium bromide + Pour agarose in gel caster + Leave casting agarose to solidify + Set up the gel and remove comb and add loading buffer 0.5 X TBE buffer + Load samples + Run the gel on 70 Voltage + Visualize the gel on gel documentation system) (Watch Related Video in #geneticteacher) #geneticteacher