Principle of CAPS and dCAPS Markers

For BLAST Sequence by NCBI.: 1- Choose the required sequence as query, 2- Select BLAST program, 3- Choose the database to search, 4- Adjust the optional parameters and click BLAST. The output of BLAST are (Graphic Display + Hit List + Alignment). In Graphic display (Red Color refer to excellent BLAST, Pink Color refer to pretty good BLAST, Green Color refer to OK BLAST, Blue Color refer to Bad BLAST, Black Color refer to really bad BLAST, Longer matched sequence segments refer to better BLAST. In addition BLAST Hit List provides accession number that links to gene bank and genome link and expected value as indicator of how good match to query sequence and score that refer to link alignment (Watch Related Video in #geneticteacher) #geneticteacher