Functions of Taq DNA Polymerase in PCR Reaction

Mechanisms of HPLC: 1- Sample is introduced through column, 2- Sample components moves to column with high pressure delivered from pump for separating, 3- Separated components detects by detector to measure their accounts in chromatogram. Therefore, 1- Sample size must be tiny (3-5) micrometer in diameter, 2- Internal diameter of column must be small with high sensitivity, 3- Pump pressure must be high for high separation, 4- Temperature must be high for high separation #geneticteacher