Functions of Taq DNA Polymerase in PCR Reaction

Enzyme Linked Immunosorbent Assay (ELISA-Plate) have polystyrene plate (96 or 384 wells) and require microliter quantities, so, it widely used in immunoassay by immobilize antigen to sild surface and linked complexes antigen/antibody to an enzyme. Therefore, detection is accomplished by assessing the conjugated enzyme activity by incubation with substrate to produce measurable product at 450 nanometers. So, during use ELISA-Plate use 50 microliters positive control (+) in first three wells and use 50 microliters negative control (-) in second three wells and use 50 microliters each samples in duplicate or triplet in remaining wells #geneticteacher